-
Product Name
USP1 antibody
- Documents
-
Description
USP1 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells. Positive IHC detected in human cervical cancer tissue. Observed molecular weight by Western-blot: 110kd, 100kd
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
Deubiquitinating enzyme 1 antibody; hUBP antibody; ubiquitin specific peptidase 1 antibody; Ubiquitin thiolesterase 1 antibody; UBP antibody; USP1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of USP1 recombinant protein (Accession Number: NM_001017416). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
-
Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116585(USP1 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:116585(USP1 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:116585(USP1 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-USP1 (IP:Catalog No:116585, 3ug; Detection:Catalog No:116585 1:300) with HEK-293 cells lysate 3200ug.
-
Background
USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).
-
References
- Ciccia A, Nimonkar AV, Hu Y. Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress. Molecular cell. 47(3):396-409. 2012.
- Liang Q, Dexheimer TS, Zhang P. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nature chemical biology. 10(4):298-304. 2014.
- Xiao H, Tian Y, Yang Y. USP22 acts as an oncogene by regulating the stability of cyclooxygenase-2 in non-small cell lung cancer. Biochemical and biophysical research communications. 460(3):703-8. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"