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Product Name
Ubiquilin 1 antibody
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Description
Ubiquilin 1 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IHC detected in human pancreas tissue, human gliomas tissue. Positive WB detected in rat brain tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 71 kDa
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Tested applications
ELISA, IHC, WB, IF
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
DA41 antibody; DSK2 antibody; FLJ90054 antibody; hPLIC 1 antibody; PLIC 1 antibody; PLIC1 antibody; ubiquilin 1 antibody; UBQLN1 antibody; XDRP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Ubiquilin 1 recombinant protein (Accession Number: NM_053067). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116648(Ubiquilin 1 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human pancreas slide using Catalog No:116648(UBQLN1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human gliomas slide using Catalog No:116648(UBQLN1 Antibody) at dilution of 1:50
Immunofluorescent analysis of PC-3 cells using Catalog No:116648(Ubiquilin 1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"