Mouse TRAIL R2/CD262/TNFRSF10B (His Tag) recombinant protein

Mouse TRAIL R2/CD262/TNFRSF10B (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis (By similarity).

Tumor necrosis factor receptor superfamily member 10B

TRICKB; TRICK2; LY98; DR5; TNFRSF10B; MK; TRAIL R2; CD262; KILLER; TRAIL-R2; TRICK2A; TRAILR2; TRICK2B; ZTNFR9; KILLER/DR5

Q9QZM4

Tnfrsf10b; Dr5; Killer

Human Cells

A DNA sequence encoding the extracellular domain of mouse TNFRSF10B (NP_064671.2) (Met 1-Ser 177) was expressed, with a C-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse TNFRSF10B consists of 136 amino acids and has a calculated molecular mass of 15 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 25-35 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Immobilized mouse TNFRSF10B-His at 10 μg/ml (100 μl/well) can bind biotinylated human TNFSF10 (Cat:501935), The EC₅₀ of biotinylated human TNFSF10 (Cat:501935) is 0.16-0.38 μg/ml.
2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is typically 0.5-2 μg/mL in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10.