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Product Name
Human RANKL/OPGL/TNFSF11 (CD254) recombinant protein
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Description
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
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Protein name
Tumor necrosis factor ligand superfamily member 11
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Protein short names
SODF; ODF; RANKL; CD254; OPGL; TNFSF11; OPTB2; OPG; LY109L; TRANCE; HRANKL2; RP11-86N24.2
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Uniprot ID
O14788
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Gene Name
TNFSF11; OPGL; RANKL; TRANCE
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human TNFSF11 (O14788-2) (Gly 63-Asp 244) was expressed.
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Protein Species
Human
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Molecular weight
The recombinant human TNFSF11 consists of 182 amino acids and has a calculated molecular mass of 20.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 27 kDa due to the glycosylation.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
1. Immobilized human TNFSF11 at 10 μg/ml (100 μl/well) can bind biotinylated human TNFRSF11B-His (Cat:500115), The EC50 of biotinylated human TNFRSF11B-His (Cat:500115) is 5.7-13.4 ng/ml.
2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 1.5-7.5 ng/mL. -
Validations
Human RANKL / OPGL / TNFSF11 / CD254 Protein SDS-PAGE
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