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Product Name
transgelin/SM22 antibody
- Documents
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Description
transgelin/SM22 Rabbit Polyclonal antibody. Positive WB detected in mouse colon tissue, human colon tissue. Positive IHC detected in human heart tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 19-22kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SM22 antibody; SM22 alpha antibody; SM22a antibody; SM22alpha antibody; TAGLN antibody; TAGLN1 antibody; transgelin antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of transgelin/SM22 recombinant protein (Accession Number: NM_003186). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:116333(TAGLN Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116333(TAGLN Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:116333(TAGLN Antibody) at dilution of 1:50 (under 40x lens)
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Background
The transgelin family is a group of proteins that belong to 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgelin 1 protein. It can cross-react with other two transgelins based on the sequence similarity.
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References
- Zhang R, Zhou L, Li Q, Liu J, Yao W, Wan H. Up-regulation of two actin-associated proteins prompts pulmonary artery smooth muscle cell migration under hypoxia. American journal of respiratory cell and molecular biology. 41(4):467-75. 2009.
- Hui S, Choi J, Zaidi S. Peptide-mediated disruption of calmodulin-cyclin E interactions inhibits proliferation of vascular smooth muscle cells and neointima formation. Circulation research. 108(9):1053-62. 2011.
- Steinbach SK, El-Mounayri O, DaCosta RS. Directed differentiation of skin-derived precursors into functional vascular smooth muscle cells. Arteriosclerosis, thrombosis, and vascular biology. 31(12):2938-48. 2011.
- Kim HJ, Kang UB, Lee H. Profiling of differentially expressed proteins in stage IV colorectal cancers with good and poor outcomes. Journal of proteomics. 75(10):2983-97. 2012.
- Moncada-Pazos A, Obaya AJ, Llamazares M. ADAMTS-12 metalloprotease is necessary for normal inflammatory response. The Journal of biological chemistry. 287(47):39554-63. 2012.
- Zhou L, Zhang R, Zhang L. Upregulation of transgelin is an independent factor predictive of poor prognosis in patients with advanced pancreatic cancer. Cancer science. 104(4):423-30. 2013.
- Meiler S, Baumer Y, Huang Z. Selenoprotein K is required for palmitoylation of CD36 in macrophages: implications in foam cell formation and atherogenesis. Journal of leukocyte biology. 93(5):771-80. 2013.
- Zhou YB, Gao Q, Li P. Adrenomedullin attenuates vascular calcification in fructose-induced insulin resistance rats. Acta physiologica (Oxford, England). 207(3):437-46. 2013.
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