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Product Name
TRAF6 antibody
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Description
TRAF6 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive IF detected in Hela cells. Positive WB detected in A431 cells, Apoptosised HeLa cells, HeLa cells, HT-1080 cells, human heart tissue, Jurkat cells, K-562 cells, mouse thymus tissue, rat heart tissue. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
RING finger protein 85 antibody; RNF85 antibody; TRAF6 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TRAF6 recombinant protein (Accession Number: NM_004620). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:116234(TRAF6 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver using Catalog No:116234(TRAF6 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:116234(TRAF6 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TRAF6 antibody Catalog No:116234 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.
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References
- Geng J, Sun X, Wang P. Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity. Nature immunology. 16(11):1142-52. 2015.
- Shi S, Liang D, Chen Y. Gx-50 reduces β-amyloid-induced TNF-α, IL-1β, NO and PGE2 expression and inhibits NF- κB signaling in a mouse model of Alzheimer's disease. European journal of immunology. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"