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Product Name
TRAF3 Polyclonal Antibody
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Description
Polyclonal antibody to TRAF3
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Tested applications
WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
TRAF3 antibody; CAP-1 antibody; CAP1 antibody; CD40bp antibody; CRAF1 antibody; IIAE5 antibody; LAP1 antibody; TNF receptor-associated factor 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human TRAF3
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100 -
Validations
Western blot - TRAF3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TRAF3 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - TRAF3 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using TRAF3 antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - TRAF3 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug TRAF3 antibody . Western blot was performed from the immunoprecipitate using TRAF3 antibody at a dilition of 1:1000.
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Background
Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"