-
Product Name
TRAF3 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to TRAF3
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
TRAF3 antibody; CAP-1 antibody; CAP1 antibody; CD40bp antibody; CRAF1 antibody; IIAE5 antibody; LAP1 antibody; TNF receptor-associated factor 3 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TRAF3 (NP_003291.2).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:200 - 1:1000
IHC 1:50 - 1:200 -
Validations
Western blot - TRAF3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TRAF3 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - TRAF3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using TRAF3 antibody at dilution of 1:100 (40x lens).
-
Background
Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"