TOP2A antibody

Cat.#: 116246

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    TOP2A antibody
  • Documents
  • Description
    TOP2A Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive IHC detected in human tonsillitis tissue, human breast cancer tissue, human testis tissue. Positive WB detected in HeLa cells, mouse testis tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 170 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    DNA topoisomerase 2 alpha antibody; TOP2 antibody; TOP2A antibody; TP2A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of TOP2A recombinant protein (Accession Number: NM_001067). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116246(TOP2A Antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116246(TOP2A Antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human tonsillitis slide using Catalog No:116246(TOP2A Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human tonsillitis slide using Catalog No:116246(TOP2A Antibody) at dilution of 1:50

    Immunofluorescent analysis of A431 cells using Catalog No:116246(TOP2A Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Immunofluorescent analysis of A431 cells using Catalog No:116246(TOP2A Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    IP Result of anti-TOP2A (IP:Catalog No:116246, 4ug; Detection:Catalog No:116246 1:1000) with HeLa cells lysate 2000ug.

    IP Result of anti-TOP2A (IP:Catalog No:116246, 4ug; Detection:Catalog No:116246 1:1000) with HeLa cells lysate 2000ug.

  • Background
    TOP2A represents topoisomerase (DNA) II alpha 170kDa
  • References
    • Liu JS, He SC, Zhang ZL. Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: a proteomic study. Oncology reports. 32(6):2635-47. 2014.
    • Wu J, Yang C, Guo C. SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells. Chemico-biological interactions. 244:94-104. 2016.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"