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Product Name
TOP2A antibody
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Description
TOP2A Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive IHC detected in human tonsillitis tissue, human breast cancer tissue, human testis tissue. Positive WB detected in HeLa cells, mouse testis tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 170 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
DNA topoisomerase 2 alpha antibody; TOP2 antibody; TOP2A antibody; TP2A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TOP2A recombinant protein (Accession Number: NM_001067). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116246(TOP2A Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis slide using Catalog No:116246(TOP2A Antibody) at dilution of 1:50
Immunofluorescent analysis of A431 cells using Catalog No:116246(TOP2A Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
IP Result of anti-TOP2A (IP:Catalog No:116246, 4ug; Detection:Catalog No:116246 1:1000) with HeLa cells lysate 2000ug.
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Background
TOP2A represents topoisomerase (DNA) II alpha 170kDa
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References
- Liu JS, He SC, Zhang ZL. Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: a proteomic study. Oncology reports. 32(6):2635-47. 2014.
- Wu J, Yang C, Guo C. SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells. Chemico-biological interactions. 244:94-104. 2016.
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