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Product Name
Anti-Top2a antibody
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Description
Rabbit polyclonal antibody to Top2a
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Tested applications
WB, ICC
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Species reactivity
Human, Mouse, Rat
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Alternative names
Top antibody; Top-2 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:1,000-1:2,000
ICC: 1:200
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Validations
Fig1: Western blot analysis on Jurkat cell lysates using anti-TOP2A rabbit polyclonal antibodies.
Fig2: ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
- Background
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References
- Identification of functional nuclear export sequences in human topoisomerase IIalpha and beta. Mirski S.E., Bielawski J.C., Cole S.P. Biochem. Biophys. Res. Commun. 306:905-911(2003)
- RECQL5 cooperates with Topoisomerase II alpha in DNA decatenation and cell cycle progression. Ramamoorthy M., Tadokoro T., Rybanska I., Ghosh A.K., Wersto R., May A., Kulikowicz T., Sykora P., Croteau D.L., Bohr V.A. Nucleic Acids Res. 40:1621-1635(2012)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"