TLE3 antibody

Cat.#: 107626

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Product Information

  • Product Name
    TLE3 antibody
  • Documents
  • Description
    TLE3 Mouse Monoclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in 293 cells, human brain tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 90 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    ESG antibody; ESG3 antibody; FLJ39460 antibody; GRG3 antibody; HsT18976 antibody; KIAA1547 antibody; TLE3 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of TLE3 recombinant protein (Accession Number: BC015729). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107626(TLE3 antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107626(TLE3 antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:107626(TLE3 Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:107626(TLE3 Antibody) at dilution of 1:50

    IP Result of anti-TLE3 (IP:Catalog No:107626, 5ug; Detection:Catalog No:107626 1:500) with HEK-293 cells lysate 1480ug.

    IP Result of anti-TLE3 (IP:Catalog No:107626, 5ug; Detection:Catalog No:107626 1:500) with HEK-293 cells lysate 1480ug.

  • Background
    TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"