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Product Name
TLE3 antibody
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Description
TLE3 Mouse Monoclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in 293 cells, human brain tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
ESG antibody; ESG3 antibody; FLJ39460 antibody; GRG3 antibody; HsT18976 antibody; KIAA1547 antibody; TLE3 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of TLE3 recombinant protein (Accession Number: BC015729). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107626(TLE3 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:107626(TLE3 Antibody) at dilution of 1:50
IP Result of anti-TLE3 (IP:Catalog No:107626, 5ug; Detection:Catalog No:107626 1:500) with HEK-293 cells lysate 1480ug.
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Background
TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )
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