Product Name

TLE3 antibody

Documents

Description

TLE3 Mouse Monoclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in 293 cells, human brain tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 90 kDa

Tested applications

ELISA, WB, IHC, IP

Species reactivity

Human; other species not tested.

Alternative names

ESG antibody; ESG3 antibody; FLJ39460 antibody; GRG3 antibody; HsT18976 antibody; KIAA1547 antibody; TLE3 antibody

Isotype

Mouse IgG1

Preparation

This antibody was obtained by immunization of TLE3 recombinant protein (Accession Number: BC015729). Purification method: Protein G purified.

Clonality

Monoclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:2000

IHC: 1:20-1:200

Validations

HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107626(TLE3 antibody) at dilution of 1:1000

Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:107626(TLE3 Antibody) at dilution of 1:50

IP Result of anti-TLE3 (IP:Catalog No:107626, 5ug; Detection:Catalog No:107626 1:500) with HEK-293 cells lysate 1480ug.

Background

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.(PMID:19309506 )

TLE3 antibody

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