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Product Name
TLE3 antibody
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Description
TLE3 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue, human breast cancer tissue. Positive IP detected in HepG2 cells. Positive WB detected in HEK-293 cells, A431 cells, HepG2 cells. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ESG antibody; ESG3 antibody; FLJ39460 antibody; GRG3 antibody; HsT18976 antibody; KIAA1547 antibody; TLE3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TLE3 recombinant protein (Accession Number: BC015729). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116070(TLE3 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung cancer slide using Catalog No:116070(TLE3 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human lung cancer slide using Catalog No:116070(TLE3 Antibody) at dilution of 1:50
IP Result of anti-TLE3 (IP:Catalog No:116070, 4ug; Detection:Catalog No:116070 1:1000) with HepG2 cells lysate 2400ug.
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Background
Transducin-like enhancer of split 3(TLE3) is a corepressor that binds to a variant of transciption factor, which mediated by CTNNB1 and TCF family members in Wnt signaling. It's suggested that effect of full-length TLE family members be regulated by association with dominant-negative AES.
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