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Product Name
TIRAP Polyclonal Antibody
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Description
Polyclonal antibody to TIRAP
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
TIRAP antibody; BACTS1 antibody; Mal antibody; MyD88-2 antibody; wyatt antibody; TIR domain containing adaptor protein antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human TIRAP (NP_001034750.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - TIRAP Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using TIRAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - TIRAP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using TIRAP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TIRAP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using TIRAP antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - TIRAP Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using TIRAP antibody at dilution of 1:100 (40x lens).
Immunofluorescence - TIRAP Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using TIRAP antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"