Cynomolgus CD2 (His Tag) recombinant protein

Cynomolgus CD2 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

T-cell surface antigen CD2, also known as T-cell surface antigen T11/Leu-5, and SRBC, is a single-pass type I membrane protein. It contains one Ig-like C2-type domain and one Ig-like V-type domain. CD2 is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). CD2 has been shown to set quantitative thresholds in T cell activation both in vivo and in vitro. Further, intracellular CD2 signaling pathways and networks are being discovered by the identification of several cytosolic tail binding proteins. CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain of CD2 is implicated in the signaling function. The complex of CD2 and CD58 also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. As a cell surface glycoprotein, CD2 expressed on most human T cells and natural killer (NK) cells and plays an important role in mediating cell adhesion in both T-lymphocytes and in signal transduction.

T-cell surface antigen CD2

FLJ46032; LY-37; SRBC; LFA-2; T11; CD2; LY37; RP4-655N15.2

Q6SZ60

CD2

Human Cells

A DNA sequence encoding the cynomolgus CD2 (NP_001182402.1) (Met1-Asp209) was expressed with a polyhistidine tag at the C-terminus.

Cynomolgus

The recombinant cynomolgus CD2 comprises 196 amino acids and has a calculated molecular mass of 22.8 KDa. The apparent molecular mass of it is approximately 35.7 KDa in SDS-PAGE.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized Cynomolgus CD2-His at 10 μg/ml (100 μl/well) can bind human CD58-Fc (Cat:501121), The EC₅₀ of human CD58-Fc (Cat:501121) is 0.04-0.10 μg/ml.