SYVN1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue. Positive IP detected in mouse kidney tissue. Positive WB detected in HEK-293 cells, HepG2 cells, MDA-MB-453s cells, mouse kidney tissue, mouse ovary tissue, mouse pancreas tissue, mouse stomach tissue, NIH/3T3 cells, Raji cells, rat stomach tissue. Observed molecular weight by Western-blot: 68-76 kDa
ELISA, WB, IHC, IP, IF
Human,Mouse,Rat; other species not tested.
HRD1 antibody; KIAA1810 antibody; Synovial apoptosis inhibitor 1 antibody; SYVN1 antibody
This antibody was obtained by immunization of SYVN1 recombinant protein (Accession Number: BC030530). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115963(HRD1 antibody) at dilution of 1:1000
IP Result of anti-HRD1 (IP:Catalog No:115963, 4ug; Detection:Catalog No:115963 1:1000) with mouse kidney tissue lysate 7200ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115963(HRD1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:115963(HRD1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:115963(HRD1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
HRD1is also named as SYVN1(Synovial apoptosis inhibitor 1) , KIAA1810. It acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Two distinct binding sites mediate Hrd1 dimerization or oligomerization, one located within the transmembrane region and another within the cytosolic domain. (PMID:19864457).Western blot analysis detected abundant HRD1 expression in liver and kidney. Mouse liver and spleen expressed Hrd1 as an 85-kD protein(PMID:12646171).
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- Bernardi KM, Williams JM, Inoue T, Schultz A, Tsai B. A deubiquitinase negatively regulates retro-translocation of nonubiquitinated substrates. Molecular biology of the cell. 24(22):3545-56. 2013.
- Walczak CP, Ravindran MS, Inoue T, Tsai B. A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum. PLoS pathogens. 10(3):e1004007. 2014.
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