Mouse SIGIRR/TIR8 (His & Fc Tag) recombinant protein

Mouse SIGIRR/TIR8 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Single Ig IL-1-related receptor (SIGIRR) or TIR8 is a member of Toll-like receptor-interleukin 1 receptor signaling (TLR-IL-1R) receptor superfamily. Although SIGIRR/TIR8 shows the typical conserved motifs that characterize the IL-1R and Toll superfamily, it is structurally and functionally distinct from both. SIGIRR/TIR8 has only one Ig domain in its extracellular portion whereas the IL-1R family contains three Ig folds. An unusually long cytoplasmic domain is reminiscent of the structure of drosophila Toll, yet the SIGIRR peptide sequence is more closely related to IL-1RI. SIGIRR/TIR8 was mainly expressed in mouse and human epithelial tissues such as kidney, lung and gut. Resting and activated T and B lymphocytes and monocytes-macrophages expressed little or no SIGIRR/TIR8, with the exception of the mouse GG2EE macrophage line. Inflammation is enhanced in SIGIRR-deficient mice. SIGIRR negatively modulates immune responses. Inflammation is enhanced in SIGIRR-deficient mice, as shown by their enhanced chemokine induction after IL-1 injection and reduced threshold for lethal endotoxin challenge.

Single immunoglobulin and toll-interleukin 1 receptor (TIR) domain, isoform CRA_d

PRO342; UNQ301; MGC110992; AI256711; TIR8; MGC102426

Q9JLZ8

Sigirr; AI256711; mCG_22567

Human Cells

A DNA sequence encoding the mouse SIGIRR (NP_075546.2) (Met 1-His 117) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse SIGIRR is a disulfide-linked homodimeric protein. The reduced monomer consists of 365 amino acids and has a predicted molecular mass of 40.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmSIGIRR/Fc monomer is approximately 60-65 kDa due to glycosylation.

> 95 % as determined by SDS-PAGE