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Product Name
RENALASE antibody
- Documents
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Description
RENALASE Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, mouse small intestine tissue. Positive IP detected in HEK-293 cells. Positive IHC detected in human kidney tissue. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C10orf59 antibody; FLJ11218 antibody; MAO C antibody; Monoamine oxidase C antibody; RENALASE antibody; RNLS antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RENALASE recombinant protein (Accession Number: NM_001031709). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:114688(RENALASE antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:114688(RENALASE antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:114688(RENALASE antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RENALASE (IP:Catalog No:114688, 4ug; Detection:Catalog No:114688 1:500) with HEK-293 cells lysate 2000ug.
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Background
RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.
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References
- Wu Y, Xu J, Velazquez H. Renalase deficiency aggravates ischemic myocardial damage. Kidney international. 79(8):853-60. 2011.
- Quelhas-Santos J, Soares-Silva I, Fernandes-Cerqueira C. Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients. Experimental biology and medicine (Maywood, N.J.). 239(4):502-8. 2014.
- Xu J, Li G, Wang P. Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure. The Journal of clinical investigation. 115(5):1275-80. 2005.
- Li G, Xu J, Wang P. Catecholamines regulate the activity, secretion, and synthesis of renalase. Circulation. 117(10):1277-82. 2008.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"