RBBP7 Polyclonal Antibody

Cat.#: 161639

Special Price 174.2 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    RBBP7 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to RBBP7
  • Tested applications
    WB, IHC, IF, ChIP
  • Species reactivity
    Human, Rat
  • Alternative names
    RBBP7 antibody; RbAp46 antibody; histone-binding protein RBBP7 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RBBP7 (NP_002884.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:200
    ChIP 1:50 - 1:200
  • Validations

    Western blot - RBBP7 Polyclonal Antibody

    Western blot - RBBP7 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using RBBP7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

    Immunohistochemistry - RBBP7 Polyclonal Antibody

    Immunohistochemistry - RBBP7 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded human gastric cancer using RBBP7 antibody at dilution of 1:100 (40x lens).

    Immunofluorescence - RBBP7 Polyclonal Antibody

    Immunofluorescence - RBBP7 Polyclonal Antibody

    Immunofluorescence analysis of MCF7 cells using RBBP7 antibody . Blue: DAPI for nuclear staining.

    Chromatin Immunoprecipitation - RBBP7 Polyclonal Antibody

    Chromatin Immunoprecipitation - RBBP7 Polyclonal Antibody

    Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP7 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

  • Background
    Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"