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Product Name
RBBP7 Polyclonal Antibody
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Description
Polyclonal antibody to RBBP7
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Tested applications
WB, IF, IP, ChIP
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Species reactivity
Human, Mouse, Rat
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Alternative names
RBBP7 antibody; RbAp46 antibody; histone-binding protein RBBP7 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human RBBP7 (NP_002884.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200
ChIP 1:50 - 1:200 -
Validations
Western blot - RBBP7 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using RBBP7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunofluorescence - RBBP7 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using RBBP7 antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - RBBP7 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug RBBP7 antibody . Western blot was performed from the immunoprecipitate using RBBP7 antibody at a dilition of 1:1000.
Chromatin Immunoprecipitation - RBBP7 Polyclonal Antibody
Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP7 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Background
Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the type B histone acetyltransferase (HAT) complex, which is required for chromatin assembly following DNA replication; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; and the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"