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Product Name
Rat IL23 (IL23A & IL12B Heterodimer) recombinant protein
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Uniprot ID
Q91Z84
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the rat IL23A (NP_569094.1) (Met1-Ala196) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rat IL12B (EDM04146.1) (Met1-Ser335) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the rat IL23A & the rat IL12B heterodimer was purified.
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Protein Species
Rat
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Molecular weight
The recombinant rat IL23A & the rat IL12B comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.2 (21+37.2) KDa. The apparent molecular mass of the rat IL23A & the rat IL12B heterodimer is approximately 47.08, 43.29, 27.67 and 24.75 KDa respectively in SDS-PAGE.
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Purity
(17.9+53.5+2.9+23.8) % as determined by SDS-PAGE
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Activity
Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 2-15 ng/mL.
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Validations
Rat IL-23 (IL23A & IL12B Heterodimer) Protein SDS-PAGE
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"