• Mouse IL23 (IL23A & IL12B Heterodimer) recombinant protein

Mouse IL23 (IL23A & IL12B Heterodimer) recombinant protein

Cat.#: 504794

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Product Information

  • Product Name
    Mouse IL23 (IL23A & IL12B Heterodimer) recombinant protein
  • Documents
  • Uniprot ID
    Q9EQ14
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the mouse IL23A (Q9EQ14) (Met1-Ala196 ) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding mouse IL12B (P43432) (Met1-Ser335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse IL23 heterodimer was purified.
  • Protein Species
    Mouse
  • Molecular weight
    The recombinant mouse IL23 (mouse IL23A&IL12B Heterodimer) comprises 510 (186+324) amino acids and has a calculated molecular mass of 58.3 (21.1+ 37.2) KDa. The apparent molecular mass of the recombinant protein is approximately 47,45 and 26 KDa respectively in SDS-PAGE under reducing conditions.
  • Purity
    (33.1+31.5+32.9) % as determined by SDS-PAGE
  • Activity
    Measured by its binding ability in a functional ELISA.
    1. Measured by its ability to bind recombinant rat IL23R-Fc (Cat:504727) in a functional ELISA.
    2. Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind human IL23R-Fc (Cat:504738). The EC50 of human IL23R-Fc (Cat:504738) is 0.14-0.35 μg/ml.
    3.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc (Cat:502630). The EC50 of Cynomolgus IL23R-Fc (Cat:502630) is 0.05-0.11 μg/ml.
    4.Immobilized mouse IL12Bh (m)+mIL23Ah at 10 μg/ml (100 μl/well) can bind biotinylated human IL12RB1-His (Cat:501974). The EC50 of biotinylated human IL12RB1-His (Cat:501974) is 21.3-49.8 ng/mL.
    5. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 1-5 ng/mL.
  • Validations

    Mouse IL-23 (IL23A & IL12B Heterodimer) Protein SDS-PAGE

    Mouse IL-23 (IL23A & IL12B Heterodimer) Protein SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"