Rat GFRA1 (Fc Tag) recombinant protein

Rat GFRA1 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-β (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.

GDNF family receptor alpha-1

GFRA1; DKFZP313E1012; RETL1; AU042498; MGC23045; RET1L; DKFZP686J0156; FLJ31546; FLJ10561; GFR-ALPHA-1; GDNFRA; TRNR1; GDNFR

Q62997

Gfra1; Gdnfra; Retl1; Trnr1

Human Cells

A DNA sequence encoding the rat GFRA1 (Q62997) (Met1-Leu445) was expressed with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat GFRA1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 662 amino acids and has a predicted molecular mass of 73.8 kDa. The apparent molecular mass of the protein is approximately 85 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Immobilized rat His-GDNF (78-211) (Cat:501013) at 10 μg/ml (100 μl/well) can bind rat GFRA1-Fc, The EC₅₀ of rat GFRA1-Fc is 20-46.6 ng/ml.