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Product Name
GFRA1 Polyclonal Antibody
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Description
Polyclonal antibody to GFRA1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
GFRA1 antibody; GDNFR antibody; GDNFRA antibody; GFR-ALPHA-1 antibody; RET1L antibody; RETL1 antibody; TRNR1 antibody; GDNF family receptor alpha-1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 of human GFRA1 (NP_665736.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - GFRA1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GFRA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - GFRA1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using GFRA1 antibody . Blue: DAPI for nuclear staining.
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Background
Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"