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Product Name
RAIDD antibody
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Description
RAIDD Rabbit Polyclonal antibody. Positive IP detected in mouse kidney tissue. Positive WB detected in K-562 cells, HEK-293 cells, HeLa cells, MCF7 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, mouse testis tissue, Raji cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 23 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CRADD antibody; RAIDD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RAIDD recombinant protein (Accession Number: NM_003805). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114528(CRADD antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:114528(CRADD antibody) at dilution of 1:100 (under 25x lens)
IP Result of anti-CRADD (IP:Catalog No:114528, 3ug; Detection:Catalog No:114528 1:1000) with mouse kidney tissue lysate 4000ug.
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Background
RAIDD, also named as CRADD, is an apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. CRADD interacts with BCL10 through its caspase recruitment domain and suppresses interactions between BCL10 and CARMA1. It is a negative regulator of the CARMA1 signalosome and suppressor of Th1-and Th17-mediated inflammatory responses.
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References
- Lin Q, Liu Y, Moore DJ. Cutting edge: the "death" adaptor CRADD/RAIDD targets BCL10 and suppresses agonist-induced cytokine expression in T lymphocytes. Journal of immunology (Baltimore, Md. : 1950). 188(6):2493-7. 2012.
- Qiao H, Liu Y, Veach RA, Wylezinski L, Hawiger J. The adaptor CRADD/RAIDD controls activation of endothelial cells by proinflammatory stimuli. The Journal of biological chemistry. 289(32):21973-83. 2014.
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