-
Product Name
PSMD4 antibody
- Documents
-
Description
PSMD4 Mouse Monoclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 50 kDa
-
Tested applications
ELISA, WB, IF, IHC
-
Species reactivity
Human; other species not tested.
-
Alternative names
AF 1 antibody; Antisecretory factor 1 antibody; ASF antibody; MCB1 antibody; PSMD4 antibody; pUB R5 antibody; Rpn10 antibody; S5A antibody
-
Isotype
Mouse IgG1
-
Preparation
This antibody was obtained by immunization of PSMD4 recombinant protein (Accession Number: NM_002810). Purification method: Protein G purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107489(PSMD4 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107489(PSMD4 Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:107489(PSMD4 Antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:107489(PSMD4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
-
Background
SMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"