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Product Name
PSMD4 antibody
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Description
PSMD4 Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human lung cancer tissue, human testis tissue. Positive WB detected in K-562 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, Raji cells, rat spleen tissue, RAW 264.7 cells. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IHC, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AF 1 antibody; Antisecretory factor 1 antibody; ASF antibody; MCB1 antibody; PSMD4 antibody; pUB R5 antibody; Rpn10 antibody; S5A antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSMD4 recombinant protein (Accession Number: NM_002810). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114400(PSMD4 antibody) at dilution of 1:3000
Immunohistochemistry of paraffin-embedded human lung cancer slide using Catalog No:114400(PSMD4 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human lung cancer slide using Catalog No:114400(PSMD4 Antibody) at dilution of 1:50
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:114400(PSMD4 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug PSMD4 antibody (Catalog No:114400, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.
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References
- Nanduri P, Hao R, Fitzpatrick T, Yao TP. Chaperone-mediated 26S proteasome remodeling facilitates free K63 ubiquitin chain production and aggresome clearance. The Journal of biological chemistry. 290(15):9455-64. 2015.
- Gerhardt C, Lier JM, Burmühl S. The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium. The Journal of cell biology. 210(1):115-33. 2015.
- Tomaić V, Ganti K, Pim D, Bauer C, Blattner C, Banks L. Interaction of HPV E6 oncoproteins with specific proteasomal subunits. Virology. 446(1-2):389-96. 2013.
- Sparks A, Dayal S, Das J, Robertson P, Menendez S, Saville MK. The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a. Oncogene. 33(38):4685-96. 2014.
- Wang Z, Fan C, Zhou HF. S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway. Journal of cell science. 127(Pt 15):3257-68. 2014.
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