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Product Name
PSAP antibody
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Description
PSAP Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, MCF7 cells, mouse brain tissue. Positive IHC detected in human lung cancer tissue, human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
A1 activator antibody; Cerebroside sulfate activator antibody; Co beta glucosidase antibody; Component C antibody; CSAct antibody; Dispersin antibody; FLJ00245 antibody; GLBA antibody; Glucosylceramidase activator antibody; Proactivator polypeptide antibody; prosaposin antibody; Protein C antibody; PSAP antibody; SAP 1 antibody; SAP 2 Saposin D antibody; SAP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PSAP recombinant protein (Accession Number: NM_002778). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:114287(PSAP antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:114287(PSAP antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:114287(PSAP antibody) at dilution of 1:200 (under 40x lens)
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Background
The PSAP gene encodes prosaposin, a precursor of four small nonenzymatic glycoproteins termed 'sphingolipid activator proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A is encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. Saposins A-D are required for the hydrolysis of certain sphingolipids by specific lysosomal hydrolases. (PMID: 2001789) Defects in PSAP are the cause of Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy (PubMed: 2060627, PMID: 15773042). This PSAP antibody (10801-1-AP) is expected to recognize both saposin A and B. Read more about this antibody on the blog:http://blog.ptglab.com/index.php/psap-antibody-uncovers-potential-role-of-prosaposin-in-cancer-survival/
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References
- Li N, Sarojini H, An J, Wang E. Prosaposin in the secretome of marrow stroma-derived neural progenitor cells protects neural cells from apoptotic death. Journal of neurochemistry. 112(6):1527-38. 2010.
- Costain WJ, Haqqani AS, Rasquinha I. Proteomic analysis of synaptosomal protein expression reveals that cerebral ischemia alters lysosomal Psap processing. Proteomics. 10(18):3272-91. 2010.
- Koochekpour S, Hu S, Vellasco-Gonzalez C. Serum prosaposin levels are increased in patients with advanced prostate cancer. The Prostate. 72(3):253-69. 2012.
- Wu Y, Sun L, Zou W. Prosaposin, a regulator of estrogen receptor alpha, promotes breast cancer growth. Cancer science. 103(10):1820-5. 2012.
- Turtoi A, Musmeci D, Naccarato AG. Sparc-like protein 1 is a new marker of human glioma progression. Journal of proteome research. 11(10):5011-21. 2012.
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