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Product Name
PRSS8 antibody
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Description
PRSS8 Rabbit Polyclonal antibody. Positive WB detected in human colon tissue, mouse kidney tissue. Positive IHC detected in human prostate cancer tissue, human ovary tumor tissue. Observed molecular weight by Western-blot: 39 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CAP1 antibody; PROSTASIN antibody; protease antibody; serine antibody; PRSS8 antibody; Serine protease 8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PRSS8 recombinant protein (Accession Number: NM_002773). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:114281(PRSS8 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114281(PRSS8 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:114281(PRSS8 antibody) at dilution of 1:50 (under 40x lens)
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Background
PRSS8(protease, serine, 8) displays trypsin-like enzymatic activities by hydrolyzing peptidyl fluorogenic substrates such as D-Pro-Phe-Arg-AMC. This trypsinlike enzymatic activity can be inhibited by aprotinin, antipain, leupeptin, and benzamidine(PMID:11274175). It is a heterodimer of two chains, light and heavy, which are held by a disulfide bond. PRSS8 is also named as CAP1 and belongs to the peptidase S1 family.
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References
- Steensgaard M, Svenningsen P, Tinning AR. Apical serine protease activity is necessary for assembly of a high-resistance renal collecting duct epithelium. Acta physiologica (Oxford, England). 200(4):347-59. 2010.
- Yang Y, Zhang J, Gong Y. Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion. Journal of perinatology : official journal of the California Perinatal Association. 35(1):16-22. 2015.
- Andersen H, Friis UG, Hansen PB, Svenningsen P, Henriksen JE, Jensen BL. Diabetic nephropathy is associated with increased urine excretion of proteases plasmin, prostasin and urokinase and activation of amiloride-sensitive current in collecting duct cells. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. 30(5):781-9. 2015.
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