Rat IL-25 (Fc Tag) recombinant protein

Rat IL-25 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17-producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)-activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.

Protein Il25

RATTUS NORVEGICUS; RGD1561632; IL-17E; IL17E; UNQ3120; PRO10272

D3ZLB1

Il25

Human Cells

A DNA sequence encoding the rat IL25 (D3ZLB1) (Val17-Ala169) was expressed, fused with Fc region of human IgG1 at the N-terminus.

Rat

The recombinant rat IL25 comprises 413 amino acids and predicts a molecular mass of 46.1 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 85 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized mouse IL17BR-His (Cat:502256) at 10 μg/mL (100 μl/well) can bind rat Fc-IL25 (Cat:500018). The EC₅₀ of rat Fc-IL25 is 0.12-0.27 μg/mL.