Mouse IL-25 (His Tag) recombinant protein

Mouse IL-25 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17-producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)-activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.

IL25

RGD1561632; RATTUS NORVEGICUS; IL17E; IL-17E; PRO10272; UNQ3120

Q8VHH8

Il25; Il17e

Human Cells

A DNA sequence encoding the mature form of mouse IL25 (NP_542767.1) (Val 17-Ala 169) was expressed with a N-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse IL25 consists of 175 amino acids and has a calculated molecular mass of 20 kDa. As a result of glycosylation, rmIL25 migrates with the MW of approximately 30-35 kDa in SDS-PAGE under reducing conditions.

> 80 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized mouse IL25-His (Cat：50138-M07H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse IL17BR-His (Cat:502256) with a linear range of 3.125-50 ng/mL.