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Product Name
IL25 Polyclonal Antibody
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Description
Polyclonal antibody to IL25
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
IL25 antibody; IL17E antibody; interleukin-25 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-177 of human IL25 (NP_073626.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200 -
Validations
Western blot - IL25 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IL25 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 60s.
Immunohistochemistry - IL25 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using IL25 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - IL25 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using IL25 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - IL25 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using IL25 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - IL25 Polyclonal Antibody
Immunofluorescence analysis of human lung cancer cells using IL25 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - IL25 Polyclonal Antibody
Immunofluorescence analysis of rat bronchus cells using IL25 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - IL25 Polyclonal Antibody
Immunofluorescence analysis of mouse lung cells using IL25 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"