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Product Name
PPIF-Specific antibody
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Description
PPIF-Specific Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in human heart tissue, HepG2 cells, mouse brain tissue, rat heart tissue. Positive IHC detected in human heart tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cyclophilin F antibody; Cyp D antibody; CYP3 antibody; FLJ90798 antibody; peptidylprolyl isomerase F antibody; PPIase F antibody; PPIF antibody; Rotamase F antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPIF-Specific recombinant protein (Accession Number: NM_005729). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:114124(PPIF-Specific antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human heart using Catalog No:114124(PPIF-Specific antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:114124(PPIF-Specific antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using PPIF antibody Catalog No:114124 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PPIF-Specific (IP:Catalog No:114124, 3ug; Detection:Catalog No:114124 1:1000) with mouse heart tissue lysate 4000ug.
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Background
PPIF, also named as CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF
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References
- Wang YE, Marinov GK, Wold BJ, Chan DC. Genome-wide analysis reveals coating of the mitochondrial genome by TFAM. PloS one. 8(8):e74513. 2013.
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