NovoPro
Cart 2
  • Phospho-MAPKAPK2-T334 pAb
  • Phospho-MAPKAPK2-T334 pAb
  • Phospho-MAPKAPK2-T334 pAb

Phospho-MAPKAPK2-T334 pAb

Cat.#: 166427

Special Price 232.3 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Phospho-MAPKAPK2-T334 pAb
  • Documents
  • Description
    Polyclonal antibody to Phospho-MAPKAPK2-T334
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    MAPKAPK2 antibody; MAPKAP-K2 antibody; MK-2 antibody; MK2 antibody; MAP kinase-activated protein kinase 2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A phospho specific peptide corresponding to residues surrounding T334 of human MAPKAPK2
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:100
    IF 1:100 - 1:200
  • Validations

    Western blot - Phospho-MAPKAPK2-T334 pAb

    Western blot - Phospho-MAPKAPK2-T334 pAb

    Western blot analysis of extracts from HeLa cells, using Phospho-MAPKAPK2-T334 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.

    Immunohistochemistry - Phospho-MAPKAPK2-T334 pAb

    Immunohistochemistry - Phospho-MAPKAPK2-T334 pAb

    Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-MAPKAPK2-T334 antibody .

    Immunofluorescence - Phospho-MAPKAPK2-T334 pAb

    Immunofluorescence - Phospho-MAPKAPK2-T334 pAb

    Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-MAPKAPK2-T334 antibody .

  • Background
    Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"