Polyclonal antibody to Phospho-GRIN2B-Y1474
Human, Mouse, Rat
GRIN2B antibody; EIEE27 antibody; GluN2B antibody; MRD6 antibody; NMDAR2B antibody; NR2B antibody; hNR3 antibody; glutamate receptor ionotropic, NMDA 2B antibody
Antigen: A phospho specific peptide corresponding to residues surrounding Y1474 of human GRIN2B
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IF 1:100 - 1:200
Western blot - Phospho-GRIN2B-Y1474 pAb
Western blot analysis of extracts from A431 cells, using Phospho-GRIN2B-Y1474 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunofluorescence - Phospho-GRIN2B-Y1474 pAb
Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-GRIN2B-Y1474 antibody .
Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (By similarity).
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