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Product Name
Phospho-FOXO4-S197 pAb
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Description
Polyclonal antibody to Phospho-FOXO4-S197
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
FOXO4 antibody; AFX antibody; AFX1 antibody; MLLT7 antibody; forkhead box O4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A phospho specific peptide corresponding to residues surrounding S197 of human FOXO4
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:100 - 1:200 -
Validations
Western blot - Phospho-FOXO4-S197 pAb
Western blot analysis of extracts from 293 cells using phospho-FOXO4-S197 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-FOXO4-S197 pAb
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-FOXO4-S197 antibody .
Immunofluorescence - Phospho-FOXO4-S197 pAb
Immunofluorescence analysis of methanol-fixed MCF-7 cells using Phospho-FOXO4-S197 antibody .
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Background
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
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