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Product Name
PHGDH antibody
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Description
PHGDH Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells. Positive IP detected in mouse brain tissue. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 57 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
3 PGDH antibody; 3PGDH antibody; PDG antibody; PGAD antibody; PGD antibody; PGDH antibody; PGDH3 antibody; PHGDH antibody; phosphoglycerate dehydrogenase antibody; SERA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PHGDH recombinant protein (Accession Number: NM_006623). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113816(PHGDH antibody) at dilution of 1:1200
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:113816(PHGDH antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:113816(PHGDH antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-PHGDH (IP:Catalog No:113816, 4ug; Detection:Catalog No:113816 1:1000) with mouse brain tissue lysate 4000ug.
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Background
PHGDH(D-3-phosphoglycerate dehydrogenase) is also named as 3-PGDH, PGDH3 and belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. It catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. 3-PGDH deficiency is a rare recessive inborn error in the biosynthesis of the amino acid L-serine characterized clinically by congenital microcephaly, psychomotor retardation, and intractable seizures(PMID:19235232 ).
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References
- Ost M, Keipert S, van Schothorst EM. Muscle mitohormesis promotes cellular survival via serine/glycine pathway flux. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 29(4):1314-28. 2015.
- Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"