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Product Name
Anti-PGDH/PHGDH antibody
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Description
Rabbit polyclonal to PGDH/PHGDH
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Tested applications
ELISA, WB, IP
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Species reactivity
Human PGDH / PHGDH
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Alternative names
NLS1 antibody; PGD antibody; PGD antibody; 3PGDH antibody; 3PGDH antibody; 3-PGDH antibody; 3-PGDH antibody; 3-phosphoglycerate dehydrogenase antibody; 3-phosphoglycerate dehyrogenase antibody; 4930479N23 antibody; A10 antibody; D-3-phosphoglycerate dehydrogenase antibody; D-3-phosphoglycerate dehydrogenase antibody; PDG antibody; PGAD antibody; PGAD antibody; PGDH antibody; PGDH antibody; PGDH antibody; RP4-683H9.1 antibody; SERA antibody; SERA antibody; NLS antibody; PDG antibody; PGD antibody; PGAD antibody; SERA antibody; 3PGDH antibody; 3-PGDH antibody; PHGDHD antibody; HEL-S-113 antibody; A10 antibody; PGD antibody; PGAD antibody; PGDH antibody; SERA antibody; 3PGDH antibody; 3-PGDH antibody; 4930479N23 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human PGDH / PHGDH (rh PGDH / PHGDH; O43175; Met1-Phe533). PGDH / PHGDH specific IgG was purified by Human PGDH / PHGDH affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1-5 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human PGDH / PHGDH.
IP: 2-8 uL/mg of lysate
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Validations
PGDH / PHGDH Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
PGDH / PHGDH Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.
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References
- Pind S, et al. (2002) V490M, a common mutation in 3-phosphoglycerate dehydrogenase deficiency, causes enzyme deficiency by decreasing the yield of mature enzyme. J Biol Chem. 277 (9): 7136-43.
- Du H, et al. (2010) 3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells. Reproduction. 139 (1): 237-45.
- Possemato R, et al. (2011) Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Nature. 476 (7360): 346-50.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"