-
Product Name
Anti-PGDH/PHGDH antibody
- Documents
-
Description
Mouse monoclonal to PGDH/PHGDH
-
Tested applications
FCM, IF, ICC/IF
-
Species reactivity
Human PGDH / PHGDH
-
Alternative names
NLS1 antibody; PGD antibody; PGD antibody; 3PGDH antibody; 3PGDH antibody; 3-PGDH antibody; 3-PGDH antibody; 3-phosphoglycerate dehydrogenase antibody; 3-phosphoglycerate dehyrogenase antibody; 4930479N23 antibody; A10 antibody; D-3-phosphoglycerate dehydrogenase antibody; D-3-phosphoglycerate dehydrogenase antibody; PDG antibody; PGAD antibody; PGAD antibody; PGDH antibody; PGDH antibody; PGDH antibody; RP4-683H9.1 antibody; SERA antibody; SERA antibody; NLS antibody; PDG antibody; PGD antibody; PGAD antibody; SERA antibody; 3PGDH antibody; 3-PGDH antibody; PHGDHD antibody; HEL-S-113 antibody; A10 antibody; PGD antibody; PGAD antibody; PGDH antibody; SERA antibody; 3PGDH antibody; 3-PGDH antibody; 4930479N23 antibody
- Immunogen
-
Isotype
Mouse IgG2b
-
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PGDH / PHGDH (rh PGDH / PHGDH; O43175; Met1-Phe533). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
-
Clonality
Monoclonal
-
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
FCM: 0.5-2 μg/Test
ICC/IF: 10-25 μg/mL
-
Validations
PGDH / PHGDH Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human PHGDH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human PHGDH monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red). Positive staining was localized to cytoplasm.
PGDH / PHGDH Antibody, Mouse MAb
Flow cytometric analysis of Human PHGDH expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human PHGDH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
-
Background
PHGDH is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family. This new family consists of D-isomer-stereospecific enzymes. The conserved residues in this family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.
-
References
- Pind S, et al. (2002) V490M, a common mutation in 3-phosphoglycerate dehydrogenase deficiency, causes enzyme deficiency by decreasing the yield of mature enzyme. J Biol Chem. 277 (9): 7136-43.
- Du H, et al. (2010) 3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells. Reproduction. 139 (1): 237-45.
- Possemato R, et al. (2011) Functional genomics reveal that the serine synthesis pathway is essential in breast cancer. Nature. 476 (7360): 346-50.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"