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Product Name
PACT antibody
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Description
PACT Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate cancer tissue, human skin cancer tissue. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue, PC-12 cells. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DYT16 antibody; HSD 14 antibody; HSD14 antibody; PACT antibody; PKR assOCiated protein X antibody; PKR assOCiating protein X antibody; PRKRA antibody; RAX antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PACT recombinant protein (Accession Number: NM_003690). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113481(PRKRA antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113481(PRKRA antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PRKRA antibody Catalog No:113481 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PRKRA (IP:Catalog No:113481, 3ug; Detection:Catalog No:113481 1:700) with mouse testis tissue lysate 4000ug.
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Background
PRKRA represents protein kinase, interferon-inducible double stranded RNA dependent activator
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References
- Lugli G, Torvik VI, Larson J, Smalheiser NR. Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. Journal of neurochemistry. 106(2):650-61. 2008.
- Li S, Zhu J, Fu H. Hepato-specific microRNA-122 facilitates accumulation of newly synthesized miRNA through regulating PRKRA. Nucleic acids research. 40(2):884-91. 2012.
- Schweitzer CJ, Jagadish T, Haverland N, Ciborowski P, Belshan M. Proteomic analysis of early HIV-1 nucleoprotein complexes. Journal of proteome research. 12(2):559-72. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"