-
Product Name
P62/SQSTM1 antibody
- Documents
-
Description
P62/SQSTM1 Mouse Monoclonal antibody. Positive WB detected in L02 cells, HepG2 cells. Positive IF detected in Starvation treated HepG2 cells. Observed molecular weight by Western-blot: 62 kDa
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
A170 antibody; EBIAP antibody; ORCA antibody; OSIL antibody; p60 antibody; P62 antibody; P62;SQSTM1 antibody; p62B antibody; PDB3 antibody; sequestosome 1 antibody; SQSTM1 antibody; Ubiquitin binding protein p62 antibody; ZIP3 antibody
- Immunogen
-
Isotype
Mouse IgG2a
-
Preparation
This antibody was obtained by immunization of P62/SQSTM1 recombinant protein (Accession Number: NM_003900). Purification method: Protein A purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IF: 1:20-1:200
-
Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:107447(P62;SQSTM1 Antibody) at dilution of 1:2000
Immunofluorescent analysis of (-20oc Ethanol) fixed Starvation treated HepG2 cells using Catalog No:107447(P62;SQSTM1 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
-
Background
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.
-
References
- Gao J, Qin S, Jiang C. Parkin-induced ubiquitination of Mff promotes its association with p62/SQSTM1 during mitochondrial depolarization. Acta biochimica et biophysica Sinica. 47(7):522-9. 2015.
- Kovacs GG, van der Zee J, Hort J. Clinicopathological description of two cases with SQSTM1 gene mutation associated with frontotemporal dementia. Neuropathology : official journal of the Japanese Society of Neuropathology. 36(1):27-38. 2016.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"