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Product Name
Anti-SQSTM1/p62 Rabbit antibody
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Description
SQSTM1/p62 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human p62/SQSTM1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/5000-1/10000
ICC/IF: 1/50-1/200
FC: 1/50
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Validations
Immunofluorescent analysis of Hela cells, using p62/SQSTM1 Antibody.
Western blot analysis of p62/SQSTM1 expression in C2C12,THP-1,3T3,Jurkat and Hela cell lysates using p62/SQSTM1 antibody at 1/1000 dilution.Predicted band size:47KDa.Observed band size:62KDa.
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Background
Swiss-Prot Acc.Q13501.Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1.
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References
- Metformin prevents against oxidative stress-induced senescence in human periodontal ligament cellsNanotopography on titanium promotes osteogenesis via autophagy-mediated signaling between YAP and β-catenin
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"