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Product Name
OCT4 Polyclonal Antibody
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Description
Polyclonal antibody to OCT4
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
POU5F1 antibody; OCT3 antibody; OCT4 antibody; OTF-3 antibody; OTF3 antibody; OTF4 antibody; Oct-3 antibody; Oct-4 antibody; POU class 5 homeobox 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human OCT4
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - OCT4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using OCT4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - OCT4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human brain cancer using OCT4 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - OCT4 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using OCT4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"