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Product Name
Anti-Oct4 (3F5) Mouse antibody
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Description
Oct4 (3F5) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Mouse
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Alternative names
MGC22487;Oct 3;Oct 4;Oct-3;Oct-4;OCT3;Oct4;Octamer binding protein 3;Octamer binding protein 4;Octamer binding transcription factor 3;Octamer-binding protein 3;Octamer-binding protein 4;Octamer-binding transcription factor 3;OTF 3;OTF 4;OTF-3;OTF3;OTF4; P antibody
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000;Other 1/1000
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Validations
Western blot detection of POU5F1/OCT4 in 20ug and 40ug mESC cell lysates using POU5F1/OCT4 mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size: 45KDa.
Western blot detection of POU5F1/OCT4 in F9 cell lysates using POU5F1/OCT4 mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size: 45KDa.
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Background
Swiss-Prot Acc.P20263.Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
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References
- Primordial Dwarfism Gene Maintains Lin28 Expression to Safeguard Embryonic Stem Cells from Premature Differentiation
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