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Product Name
NSE antibody
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Description
NSE Mouse Monoclonal antibody. Positive IF detected in SH-SY5Y cells. Positive IHC detected in human brain tissue. Positive WB detected in SH-SY5Y cells, C6 cells, U-251 cells. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse, Rat; other species not tested.
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Alternative names
ENO2 antibody; Enolase 2 antibody; enolase 2 (gamma antibody; neuronal) antibody; Gamma enolase antibody; Neural enolase antibody; Neuron specific enolase antibody; NSE antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of NSE recombinant protein (Accession Number: NM_001975). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:107339(ENO2 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107339(ENO2 Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:107339(ENO2 Antibody) at dilution of 1:200 (under 40x lens)
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:107339(ENO2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. Three enolase isoenzymes, alpha, beta and gamma , phaare present in mammalian cells. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs inneural tissue during development.
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