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Product Name
Anti-NSE Rabbit antibody
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Description
NSE Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human NSE
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/5000-1/10000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
FC: 1/50
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Validations
Western blot analysis of extracts from Jurkat, THP-1 and U87MG cells using NSE Rabbit pAb at 1:1000 dilution. Predicted band size: 47kDa. Observed band size: 47kDa.
Immunohistochemical analysis of paraffin-embedded human uterus cancer, using NSE Antibody.
Immunofluorescent analysis of A431 cells, using NSE Antibody.
Western blot analysis of NSE expression in HeLa cell lysate.
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Background
Swiss-Prot Acc.P09104.ENO2 an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"