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Product Name
NPL antibody
- Documents
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Description
NPL Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive WB detected in mouse kidney tissue, Raji cells. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
c112 antibody; C1orf13 antibody; N acetylneuraminate lyase antibody; NALase antibody; NPL antibody; Sialate lyase antibody; Sialate pyruvate lyase antibody; Sialic acid aldolase antibody; Sialic acid lyase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NPL recombinant protein (Accession Number: NM_001200051). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:113217(NPL antibody) at dilution of 1:500
Immunofluorescent analysis of MCF-7 cells, using NPL antibody Catalog No:113217 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.
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