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Product Name
NFE2 antibody
- Documents
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Description
NFE2 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in K-562 cells. Positive WB detected in K-562 cells, U-937 cells. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Leucine zipper protein NF E2 antibody; NF E2 antibody; NFE2 antibody; p45 antibody; p45 NF E2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NFE2 recombinant protein (Accession Number: NM_001136023). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:113144(NFE2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113144(NFE2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using NFE2 antibody Catalog No:113144 at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-NFE2 (IP:Catalog No:113144, 4ug; Detection:Catalog No:113144 1:500) with K-562 cells lysate 3440ug.
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Background
The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. This is a rabbit polyclonal antibody raised against full-length NFE2 of human origin.
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References
- Khechaduri A, Bayeva M, Chang HC, Ardehali H. Heme levels are increased in human failing hearts. Journal of the American College of Cardiology. 61(18):1884-93. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"