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Product Name
NEDD4 antibody
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Description
NEDD4 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human breast cancer tissue, human lung cancer tissue. Positive WB detected in C6 cells, A549 cells, COS-7 cells, HEK-293 cells, HT-1080 cells. Positive IP detected in C6 cells. Observed molecular weight by Western-blot: 115 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat, Monkey; other species not tested.
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Alternative names
KIAA0093 antibody; NEDD 4 antibody; NEDD4 antibody; NEDD4 1 antibody; RPF1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NEDD4 recombinant protein (Accession Number: NM_006154). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
C6 cells were subjected to SDS PAGE followed by western blot with Catalog No:113096(NEDD4 antibody) at dilution of 1:300
IP Result of anti-NEDD4 (IP:Catalog No:113096, 4ug; Detection:Catalog No:113096 1:500) with C6 cells lysate 1600ug.
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:113096(NEDD4 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:113096(NEDD4 Antibody) at dilution of 1:50
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Background
NEDD4-1(Neural precursor cell expressed developmentally down-regulated protein 4) is also named as NEDD4 and KIAA0093. It plays an essential role for the activation of the genetic program involved in the terminal maturation stage of invariant natural killer T-cell development(PMID:21949387). And it is differentially expressed in mammary epithelial cells and plays a role in luminal lineage determination(PMID:20351249). NEDD4-1 is a key regulator of FGFR1 endocytosis and signalling during neuronal differentiation and embryonic development(PMID:21765395). This antibody can recognize NEDD4-1 and NEDD4-2.
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References
- Jiang K, Bai B, Ta Y. O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway. Biochemical and biophysical research communications. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"