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Product Name
Mouse NOTCH1 (His Tag) recombinant protein
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Protein name
Neurogenic locus notch homolog protein 1
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Protein short names
TAN1; 9930111A19RIK; AOS5; MIS6; NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1; LIN-12; MOTCH A; HN1; MAJOR TYPE A PROTEIN; RP23-306D20.12-001; AOVD1; N1; MT14
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Uniprot ID
Q01705
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Gene Name
Notch1; Motch
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed?with a C-terminal polyhistidine tag.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.
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Purity
> 82 % as determined by SDS-PAGE
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Activity
1. Measured by its binding ability in a functional ELISA.
2. Immobilized human DLL4 (cat: 503992) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 40 ng/mL.
3. Immobilized mouse DLL4-his (Cat:502366) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The EC50 of biotinylated mouse NOTCH1-his is 30 ng/mL. -
Validations
Mouse Notch-1 / NOTCH1 Protein (His Tag) SDS-PAGE
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