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Product Name
Anti-Notch1 Rabbit antibody
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Description
Notch1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Notch1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/3000
IHC: 1/50-1/200
ICC/IF: 1/100-1/500
FC: 1/200-1/500
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Validations
Immunofluorescent analysis of HeLa cells, using Notch1 Antibody .
Western blot analysis of Notch1 expression in MCF7,Jurkat,CEM and Rat Brain cell lysates using Notch1 antibody at 1/1000 dilution.Predicted band size:125KDa.Observed band size:120,300KDa.
Western blot analysis of NOTCH1 expression in HEK293 cell lysate.
Immunohistochemical analysis of paraffin-embedded human liver, using Notch1 Antibody .
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Background
Swiss-Prot Acc.P46531.Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"